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they frequently lack appropriate specificity, chemical stability, or interference resistance to cellular components [115] and they are mostly incapable of simultaneously quantifying the intricate network of interconnected redox couples (e.g., GSH/GSSG, NAD/NADH, and Trx redox states) with adequate sensitivity under physiologically relevant conditions [116]

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The specificity of the alpha-2 helix stems from the assortment of amino acids in the domain that interacts with the glycine residue of glutathione